Use of Trypsin in Preparing Subcultures of Monkey Testicular Tissue ∗

Abstract

The use of trypsin as a niethod of obtaining tissue cukture cells for transplanting purposes was introduced by Rous and Jones (1) and elaborated by Vogelaar and Erlichman (2). A detailed study of the stimulating effect of various proteolytic enzymes on tissue growth was reported by Siiiirns and Stillman (3). Several interesting observations were made by these various authors: a) Contact with 3% crude trypsin for 1 hour at 37°C did not injure rodent or avian cells. but 5% did(1); b) Following solution of the plasma clot, repeated subculture was possible (1,2); c) Cell growth was rapid and at times explosive (1,3) : d) The lag period for adult cells in tissue culture was shortened (3) : e) Similar stimulation was obtained with both crude and crystalline trypsin, chymo-trypsin, and papain, suggesting that proteolysis of growth inhihitory substances was an essential factor (3). The purpow of this report is to present B simple tryptic digest method of transplanting roller tube cultures of monkey testicle with the objective of increasing the yield of usable explants for the propagation of poliomyelitis and other viruses.