Abstract
Summary
Concentrated fractions of dry sheep pituitary tissue obtained from extracts made at 70°C with water, saturated solutions NaCl and KCl, and with 0.88 M sucrose contained the major part of the gonadotrophic activity. Fractions obtained from extracts made with saturated solutions of barium, calcium and magnesium chlorides, and 6.6 M urea at 70°C were much less active. Dialyzed soluble fraction obtained from saturated NaCl extracts made at 80°C contained little activity and those from extracts made at 90°C were essentially inactive. Extraction at 70°C with the saturated solutions and fractionation of the dialyzed extracts did not separate FSH from LH. The activity of the gonadotrophin was augmented when administered in solutions which were 0.5 saturated with NaCl and KCl.
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