Abstract
Summary
1. The results with 0.88 11 sucrose solution as the dispersion medium agree essentially with those obtained with alkaline water medium. 2. Succinoxidase activity of the mitochondria accounts for 43% of the total homogenate activity and cytochrome oxidase 47% on a wet weight basis. 3. On nitrogen basis the activities are 2-3 times greater than that of the homogenate. 4. After 2 washings the activity of both enzymes on a wet weight basis is reduced; on a nitrogen basis succinoxidase activity is reduced to 33% but the cytochrome oxidase activity is the same as in the original mitochondria. 5. Small amounts of the final supernatant fluid added to mitochondria increase the succinoxidase activity to 85% of the original homogenate. This effect does not represent an addition of the activities of the 2 fractions. 6. There is no change in intracellular succinoxidase and cytochrome oxidase activities after a period of starvation of 16 to 140 hours.
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