Abstract
Summary
It has been shown that the growth of Leptospira canicola on a chemically-defined medium lacking amino acids but supplemented with rabbit serum albumin is stimulated at certain levels of arginine, aspartic acid, glutamic acid or proline but is inhibited by any one of 16 amino acids at some concentration. The rabbit albumin could not be replaced by a mixture of amino acids simulating the amino acid composition of the albumin determined by microbiological assay. Thiamine was found to be the only vitamin essential for the growth of L. canicola. L. canicola, L. pomona, L. ballum, L. sejroe, and L. icterohaemorrhaiae could be subcultured on a simple medium containing only salts, thiamine, asparagine, and rabbit serum albumin.
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