Localization of the Enzymatic Block in Kidneys of Rats Treated with Fluoroacetate

Abstract

Summary

1. Oxidation of citrate was markedly depressed in bomogenates of kidneys from rats treated with fluoroacetate; oxidation of other substrates of the Krebs cycle, including cis-aconitate, was depressed only to a relatively small extent. 2. No inhibition of aconitase was found in these preparations when cis-aeonitate was the substrate. 3. The evidence indicated that the conversion of cis-aconitate to citrate was relatively unaffected under conditions in which the reverse reaction was strongly inhibited.