Abstract
Summary
A simple microbiological diffusion-plate assay for isonicotinyl hydrazine has been devised employing Mycobacterium butyricum ATCC No. 362 as the test organism. The method is suitable for assaying urines and other aqueous solutions containing 5 γ per ml or more of the drug. The solutions need not be sterile. The assay may be read in 18-24 hours and results are reproducible to ± 15% or closer. Results obtained by this method are in fair agreement with those obtained by the cyanogen bromide chemical assay.
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