Abstract
Summary
A method is described for the determination of carbon dioxide content of 0.1 and 0.2 ml samples of serum or plasma by adding 0.1 ml of standard acid and 0.1 ml of standard alkali and estimating the pH with a glass electrode. The values of carbon dioxide are then calculated from the amount of alkali found on an average composite curve for plasma or serum between an average initial pH at room temperature of 7.60 and the experimental final pH. Data are presented to show that the results obtained by this method agree quite well with values determined by the Van Slyke manometric method.
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