Abstract
Summary
The specificity, interfering artifacts, sensitivity, and localizability of the acid silver nitrate reaction for ascorbic acid were studied by means of test tube and slide reactions, model experiments, and histological methods, Whereas the specificity of the reduction reaction is reasonably good, interfering artifacts are numerous and are difficult to avoid. Protein and glutathione greatly reduce the sensitivity and velocity of the histochemical reaction. Gross diffusion artifacts render the test useless for localization on a cytological level.
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