Abstract
Summary
The validity of claims reporting an identity of ACTH and intermedin is questioned by experiments in which assays by the melanophore expanding test in the hypophysectomized frog and the adrenal ascorbic acid depleting test in the hypophysectomized rat were employed. The results demonstrate: 1. Lack of correlation between the 2 activities in various ACTH preparations. 2. Partial separation of intermedin and ACTH activities using a discontinuous pH gradient on cellulose. 3. Differential migration of activities during zone electrophoresis on filter paper. 4. Potentiation of intermedin and inactivation of ACTH by alkali-heat treatment. 5. Differential concentration of ACTH and intermedin activities in the anterior and intermediate lobes of the frog pituitary.
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