Abstract
Summary
1. A practical micro-bioassay technic for desoxycorticosterone acetate activity is proposed, using albino, urethra-ligated, bilaterally adrenalectomized, male rats of 150-155 g weight. 2. A calibration curve is demonstrated using desoxycorticosterone acetate as the standard hormone and the percentage sodium retained as the measure of its activity. Two μg DC A injected subcutaneously in 2 stages produce a highly significant retention of administered sodium. 3. The proposed bioassay requires 30 lapsed hours and a minimum of special technical skills and apparatus, but a high degree of sensitivity and a satisfactory degree of precision are indicated. 4. No consistent correlation was found between urine volume changes and DCA dosage levels. A significant change in urine volume was observed with 4 μg DCA relative to the control group of animals, but not with the higher or lower doses of DCA studied.
Get full access to this article
View all access options for this article.