Cultivation of Endamoeba Histolytica in Embryonic Fluids ∗

Abstract

Discussion and Summary

1. These experiments indicate that Endamoeba histolytica trophozoites can be cultured successfully in embryonic fluids. Amniotic and allantoic fluids from 9- to 13-day-old chick embryos have given the most consistent results. While growth of amebae occurred in aerobic as well as anaerobic environments, more dependable results were obtained under the latter condition. The fluids retained their ameba growth-supporting qualities after storage up to 30 days, but not after Seitz filtration. 2. The extremely low bacterial population has made large numbers of amebae available for physiologic studies. It is generally realized that bacterial interference must be minimized before metabolic studies of a fundamental nature can be undertaken.