Abstract
Summary
A strain of West Nile virus isolated by Dr. K. C. Smithburn from a patient's serum in Africa and passaged intracerebrally in Swiss albino mice has been successfully transmitted intracerebrally to 1-day-old White Leghorn chicks. The virus was carried intracerebrally through 8 serial passages in 1-day-old baby chicks. Virus-bearing brain suspensions from chicks of each passage proved to be infective for Swiss albino mice when inoculated intracerebrally. Virus from a chick-brain suspension of the 7th intracerebral passage was transmitted successfully to baby chicks by the following routes of exposure: intraperitoneal, intradermal, intramuscular, intracardiac, intralingual, intranasal and rectal. Baby chicks showed typical symptoms of West Nile disease between 5 to 10 days post-inoculation.
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