Abstract
Summary and Conclusions
Twelve guinea pigs were sensitized with dog hemoglobin. Subsequently blood specimens were taken and heparinized and a portion of these specimens mixed with the antigen. White cell counts were done immediately after mixing, and repeated at 30, 60, and 120 minutes. In 2 cases counts were also made at 180 and 240 minutes. Controls of blood and saline in the same ratio as blood and antigen were counted concomitantly on each guinea pig. Blood specimens f rom 10 non-sensitized guinea pigs were similarly collected and counted. No significant difference in the total white cell counts from sensitized or non-sensitized animals either when mixed with saline or with the hemoglobin antigen was detected. The total mean white cell count of the sensitized animals in this series was 32.7% and 34.0% above that of the non-sensitized animals in the control and experimental tubes respectively. The above findings indicate that in vitrolysis of sensitized guinea pig leucocytes by dlog hemoglobin antigen does not occur.
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