Inactivation of ll-Dehydro-17-Hydroxycorticosterone by Tissue Slices. ‡

Abstract

Summary

1. Slices of liver, spleen, and kidney incubated in Krebs solution for three hours with added cortisone caused approximately a 70% loss of cortisone activity. Blood serum and brain or muscle slices were inactive. Tissue homogenates were much less effective than slices. Incubation of the tissue-cortisone mixture with the enzyme, glucuronidase did not alter the experimental findings. 2. There was no detectable difference in the capacity of tissues of stressed or adrenalectomized animals, as compared to tissues from normal rats, to inactivate cortisone. The inactivation mechanism could not be altered by the pre-administration of progesterone, pregnenolone, testosterone, desoxycorticosterone or cortisone.

We wish to express our appreciation to Miss Beatrice Silides for her technical assistance.