Abstract
Summary
A method has been described for the quantitative extraction and spectro-photometric estimation of phenosulfon-phthalein (PSP) in renal cortex slices of the guinea pig. The effect of alterations of time of incubation, concentration of the PSP in the medium, and weight of tissue on the in vitro uptake of PSP by this preparation have been investigated. Uptake of PSP increases progressively with increases in incubation time. Increasing the concentration of PSP in the medium results in a decrease in the ratio of dye concentration in the slice to that in the medium. Ability of the slice to concentrate PSP from the medium is not markedly influenced by alterations of tissue weight between 200 and 400 mg.
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