Abstract
Summary
A method is described for the quantitative extraction and purification of small amounts of lipid with a minimum of manipulation. The acid-soluble material is removed from the tissue with cold 10% trichloracetic acid containing 0.4 M MgCl2. The lipid is removed from the residue using ethanol-ether. This extract is taken to near-dryness, re-extracted with petroleum ether-chloroform, and the latter solution washed with water and dried. The lipid thus prepared has been found to compare favorably in several respects with lipid purified according to McKibbin and Taylor(3).
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