Electron Micrographs of Newcastle Disease Virus Preparations from Chick Embryos Following Infection by Virus Propagated in the Short-tailed Shrew (Blarina brevicauda)

Abstract

Summary

(1) NDV from short-tailed shrews infected by intracerebral inoculation and intranasal instillation was 100% pathogenic for embryonated eggs in the first embryo passage. Shrews infected by these two methods of exposure showed typical Newcastle disease symptoms. The virus in the allantoic fluid of eggs inoculated with the shrew brain material from both intracerebral and intranasal series was neutralized by positive Newcastle disease serum but was not affected by normal chicken serum. (2) Electron micrographs of Newcastle disease virus preparations from chick embryos following infection by the virus propagated in the short-tailed shrew showed a predominance of tailed forms. (3) Intracerebral and intranasal serial passages of NDV in the short-tailed shrew are being continued at the present time and will be reported in a later paper.