Observations on a Relationship Between Vitamin B 12,Folic Acid and the Citrovorum Factor. ∗

Abstract

Summary

Vit. B₁₂ injected at low levels increased the PGA stored in chick liver tissue. Higher levels of injected vit. B₁₂ suppressed the liver storage of PGA. Vit. B₁₂ administered orally, increased the level of both PGA and LCF as measured by chick liver storage.

Vit. B₁₂ injected at the level of 1 γ per day significantly inhibited the conversion of PGA to LCF in PGA-vit. B12 deficient chicks. This inhibition was overcome by the in vitro addition of ascorbic acid. The oral administration of vit. B₁₂ at the level of 1 γ per day significantly increased the conversion of PGA to LCF with or in the absence of ascorbic acid, in the PGA-vit. B₁₂ deficient chick.

The possible relationships and interactions of vit. B₁₂, PGA, LCF, and ascorbic acid have been discussed.