Abstract
Summary and Conclusions
(1) CFW and dba mice were inoculated intraperitoneallv with Sarcoma 37 cells from serial intraperitoneal transfers. After formation of copious peritoneal exudate containing tumor cells and macrophages, these mice received intraperitoneally or intravenously 0.1 mc (in 2 series 0.3 mc) of radioactive colloidal gold. At various intervals (∗4 hour to 48 hours) after injection, specimens of peritoneal fluid were withdrawn from these mice and centrifuged. Radioactivity of precipitate (P) and of supernatant (S) in each specimen was determined by Geiger counter. (2) Ratio P/S was accepted as index of radioactivity distribution between macrophages and serous exudate of the peritoneal fluid. Data illustrating trends in changes of P/S in various groups of mice were plotted in graphs and discussed. These data were interpreted as the combined effect of two parallel phenomena responsible for disappearance of radioactive colloid from the peritoneal serous exudate; (a) absorption and storage of colloid particles into macrophages; (b) their diffusion into circulating blood; (3) The ratio P/S in various specimens of peritoneal fluid from the same mouse remained consistently on higher levels after (a) the use of higher doses of radioactive colloidal gold; (b) the use of dba mice and (c) the preparation with inactive (decayed) colloidal gold before treatment with radioactive gold. The effect of these factors is attributed to the increase in the number and in the activity of macrophages.
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