Abstract
Summary
Consistent mean recoveries of 97% are obtained by use of a simple procedure for the stable extraction of T-1824. Dye-tinged plasma is treated with a modern soap and then quantitatively transferred to a column of paper fiber. Dye is sorbed on the paper, rinsed with saline solution and eluted with acetone:water. The various steps including the spectrophotometric measurement can be finished in one hour. Spectral curves of eluted dye show that interference from hemolysis and lipemia is removed in the rinse. This procedure improves the versatility and certainty of the dye-hematocrit method for estimation of blood volume.
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