Inhibition of Synthesis of Vitamin B 12 and of Riboflavin by 1,2-Dichloro-4,5-diaminobenzene in Bacterial Cultures

Abstract

Summary

The amounts of vit. B₁₂ and of riboflavin which were formed during growth of Bacillus megatherium were decreased by l,2-dichloro-4,5-diaminobenzene when the latter was present in quantities insufficient to inhibit growth. l,2-Dimethyl-4,5-diamino-benzene caused stimulation of the production of vit. B₁₂. These facts have been viewed as being compatible with the previously expressed postulates that the dimethyldiamine is a precursor of riboflavin and of vit. B₁₂, and that the dichloro-analog exerts its selective toxicity, at least in part, by inhibition of biosynthetical processes in the formation of these vitamins.