Abstract
Summary
1. Suspensions of PPLO suitable for rabbit immunization and for use in agglutination tests were produced in relatively large quantities from cystine trvpticase broth. 2. Five human strains of PPLO were closely related antigenically, as measured by agglutination titers with homologous and heterologous rabbit sera. These strains failed to agglutinate in the presence of normal rabbit and human sera. A sixth strain was agglutinated in all normal human and rabbit sera. 3. Further evidence was obtained for the antigenic homogeneity of the human PPLO studied by cross-absorbing 2 antisera and 2 antigens. After such absorption no antibody activity remained for any of the 4 strains tested. 4. Sera from 18 cases of non-gonococcal urethritis failed to agglutinate suspensions of 5 human strains of PPLO. 5. Stable suspensions were produced from antigens lyophilized for at least 3 months.
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