Abstract
Summary
By means of Warburg respirometers the oxygen consumption of brain, connective tissue, heart, kidney, liver and lung from normal dba mice was measured at 48-hour intervals during a period of 18 days of storage at 4°C, in Tyrode's solution and in beef spleen extract. The respiration of all six of the normal tissues, whether stored in Tyrode's solution or in beef spleen extract, decreased during the initial 8- to 10-day period, while the same tissues, with the exception of liver, were characterized by a progressive increase in respiration during the 12- to 18-day period of storage. This increase could not be correlated with bacterial contamination or with tissue autolysis as revealed by histologic observation and amino nitrogen determination. The immediate effect of the extract was to decrease the oxygen consumption of brain, lung and heart, and, to a lower degree, of liver and connective tissue, while that of kidney was increased. The effect of beef spleen extract on dbrB adenocarcinoma in dba mice fell between the extremes of the effects on normal dba mouse tissues.
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