Presence of a Necrosin-like Fraction in the Extracts of Injured Tissue. ∗

Abstract

Conclusions

The crushing and mashing of cells from dog kidney, liver, and spleen yields by (NH₄)₂SO₄ fractionation a necrotizing substance located in the euglobulin fraction of the saline extracts. The pseudoglobulin-albumin fraction of the tissue extracts do not contain this toxic substance. This substance is thermolabile. It does not seem to differ in its biological property of inducing severe cell injury from necrosin as obtained especially from acid inflammatory canine or human exudates (1,2).