Abstract
Summary
Antibody titers produced in guinea pigs receiving Rh+ type O human red blood cells intraperitoneally were compared each week after immunization with those produced in similarly treated guinea pigs which also received varying doses of sodium salicylate for one day before drawing blood for the measurement of antibody titers and salicylate plasma levels. Rh+ type O human red blood cells were antigenic in the guinea pig. The Rh+ antigen added little to the antigenicity of the type O cells. The average Rh+ or Rh− antibody titers rose to 1:778 or 1:819 on the 4th day after immunization, fell to 1:313 or 1:136 on the 12th day and by the 20th day had fallen to 1:50, the baseline level. A more lasting circulating antibody titer was produced after a booster dose of Rh+ type O cells. It is concluded that the natural antihuman hemoagglutinins cannot be depressed by sodium salicylate. The data indicated that the depressant action of sodium salicylate on the Rh+ type O human antigen-antibody reaction in guinea pigs was limited by the toxicity of salicylates. Rh+ or Rh− titers between 1:136 and 1:819 were depressed significantly by non-toxic plasma levels of sodium salicylate (15-40 mg %); whereas Rh+ or Rh− titers of 1:1500 were not depressed even by toxic plasma levels (approximately 50 mg %).
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