Abstract
Summary
The effect of Tweens 20, 40, 60, 80 and 85, and oleic acid, upon the survival of chick embryo mesenchyme cells maintained in a completely synthetic feeding mixture has been studied over the concentration range of 0.5% to 0.00005%. Levels of 0.5% and 0.05% were definitely toxic whereas lower concentrations were not. Relatively small amounts of ethyl alcohol (0.3%) were inhibitory when used together with low concentrations of Tweens. Reduction of the alcohol content to a minimum (0.01%) makes it possible to employ the Tweens as a source of water-soluble fatty acids and also to dissolve fat-soluble compounds in aqueous synthetic feeding mixtures.
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