Comparative Tissue Explant Requirements of Columbia-SK,C(M) and M.M. Viruses,and of Theiler's Encephalomyelitis Virus. ∗

Abstract

Summary

In a fluid medium consisting of 3 cc of Simms' solution and 1.25 cc of oxserum ultrafiltrate plus 0.25 cc of selected minced tissue in glucosol solution, the Col. SK, C(M) and M.M. viruses were found to grow freely on tissue explants of brain, lung, small intestine, heart muscle, and tail from embryo mice, but not on explants of tongue skeletal muscle, kidney, liver, or spleen from the same embryos. Growth of all 3 viruses was also obtained on explants of minced 5-day chick embryos, but not to an equal degree.

The GD VII strain of Theiler's encephalomyelitis virus was carried through 15 culture passages on explants of brain and tail tissue, but failed to show well defined or continued growth on explants of other tissues and organs from embryo mice. Unlike the GD VII strain, the FA strain of Theiler's virus failed to grow on explants of tail tissue. Both strains grew on explants of chick embryo tissue, but the FA strain grew less freely than the GD VII strain, even on explants of mouse brain tissue, under the conditions described.