Protective Effect of Cattle Sera on White Mice Infected with Brucella abortus with the Aid of the Mucin Technic

Abstract

Summary

Lethal infections of B. abortus may be produced regularly in mice with the aid of the mucin technic by the injection of 0.5 cc of a 4-fold concentrated 48 hours broth culture. Cattle sera with agglutinin titres for B. abortus of 200 or higher protect the mice against the infection, when at least 0.05 to 0.02 cc of serum is administered 24 hours prior to infection. The minimal protecting dose and the minimal agglutinating serum quantity per cc are not closely linked. The serum prevents general septicemia and death, but does not prevent the formation of infectious foci in the abdominal organs.