The In Vitro Conversion of C 14 -Labeled Glucose to Fatty Acids. ∗

Abstract

Summary

1. The conversion of C¹⁴-labeled glucose to fatty acids by surviving slices of liver, kidney, and diaphragm is demonstrated.

2. The recoveries of fatty acid-C¹⁴ in the experiments carried out with liver slices were about 10 times as great as those observed with diaphragm or kidney.

3. The glucose-C¹⁴ recovered as fatty acid-C¹⁴ in the experiments with liver slices was 1/6 to 1/10 of that which had been converted to CO₂.

4. An incubation flask is described that is suitable for the collection of CO₂ evolved in tissue slice experiments from C₁₄-labeled compounds.