On the Insulin Content of Pancreatic Juice. ∗

Abstract

Summary

Commarized insulin added to fresh pancreatic juice can be extracted from the juice and its effect noted on the glucose uptake by the diaphragm in quantities as small as 0.0045 unit per milliliter juice.

In extracting pancreatic juice alone, no clear-cut demonstration of insulin effect (i.e., an increase in glucose uptake of 50-100%) could be obtained, even though the extracts of as much as 7 milliliters of fresh juice per milliliter of substrate were tested. (The 20 to 25% averaged increase of glucose uptake by pancreatic juice is not of statistical significance.)

Thus if insulin is present in pancreatic juice, the amount extractable and detectable by our methouds must be less than .005/7 or .0007 unit per milliliter of juice.