Abstract
Summary
The short column technic described is an adjunct to the chromatographic methods previously published(2) and makes avalibale a complete procedure for the preparation of certain subcellular particulates from the crude tissue to the purified fractions without recourse to the centrifuge at any stage.
Some of the advantages of the short column technic for the preliminary preparation of cytoplasmic fractions are speed, simplicity of equipment and operation, an adaptability to a controlled gas phase during manipulation, and a sharp separation of cytoplasmic elements from nuclei and viable cells.
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