Activation of Purified Prothrombin

Abstract

Summary

Purified prothrombin can be activated by dissolving it in a 25 per cent solution of sodium citrate, but the yield of thrombin is usually low. By adding a small amount of 3-chloro-4, 4'-diaminodiphenyl sulfone to the activation mixture the yield of thrombin is increased to the maximum. Several other diphenyl sulfones also increase the yield. In contrast, 3, 4, 4'-triaminodiphenyl sulfone and 2-hydroxy-4, 4'-diaminodiphenyl sulfone act as inhibitors of the activation process.