Abstract
Summary
Cholesterol in adequate amounts can be substituted for serum in liver infusion media for culture of entozoic amoebae, but the yields will be statistically heterogeneous in contrast with those obtained when cholesteryl oleate is used as a supplement. Oleic acid in optimum concentrations reinforces the action of cholesterol. It is suggested that cholesteryl oleate is not used as such but must be hydrolyzed to its constituent molecules.
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