Binding of Phenolsulfonephthalein by Tissues and the Effect of Denaturation Thereon. ∗

Abstract

Summary

1. Tissue proteins are capable of binding the anionic dye, phenolsulfonephthalein, in small amounts. All of the tissues examined bound less of the dye than did serum and different tissues appear to have different capabilities in this respect. Of the tissues tested liver bound most and pancreas least.

2. Denaturation of tissue proteins by heat or urea materially increased the ability of the tissue protein to bind the dye.

3. Native tissue proteins of the rat considerably decreased the binding of phenolsulfonephthalein by crystallized bovine albumin; this property was abolished by heat denaturation of the tissue proteins.