A New Serological Test (Inhibition Test) for Human Serum Globulin

Abstract

Summary and Conclusions

1. A new serological method of demonstrating human serum globulin has been described, based on its ability to inhibit the agglutinating action of anti-human-globulin serum for Rh-positive human red cells coated with Rh_o univalent antibody.

2. Using this technic, further evidence was obtained that the Rh antibody coating sensitized Rh-positive cells is a serum globulin.

3. With the new technic it is possible to demonstrate the presence in normal spinal fluid, and in saliva of small amounts of serum globulin, in proportion to the known concentration in these fluids. Normal human semen and urine failed to inhibit the antiglobulin serum.

4. Umbilical cord serum inhibited anti-globulin serum to the same titer as adult serum.

5. Serum from horse, ox and rabbit did not inhibit the anti-human-globulin serum, while rhesus monkey serum merely weakened its reactions.

6. The new technic may find application not only in clinical medicine, but also in forensic work for the examination of blood stains, and in studies on biochemical evolution.