Abstract
Summary
A severe anemia was induced in chicks by the intramuscular injection of phenylhydrazine hydrochloride (2.0 mg per 100 g body weight) following a depletion period on a folic acid deficient purified ration. Chicks were selected within narrow limits (2-4 g % Hb) and arranged into comparable groups to study the effect of folic acid, liver extract and vitamin B12 on the rate of hemoglobin regeneration.
Liver extract alone did not influence the rate of hemoglobin formation. When the depletion period was 21 days or longer, the combination of liver extract and folic acid caused a more rapid regeneration of hemoglobin than treatment with a similar dosage of folic acid alone. Pure vitamin B12 completely replaced liver extract in stimulating the formation of hemoglobin in the presence of folic acid.
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