Abstract
This paper is a report of results in continuation of those read at the February meeting. Of especial interest are the results obtained with the blood serum of three tubercular calves which showed very slight lesions at autopsy. We obtained results several months ago which led us to believe that dogs sensitized with tuberculin would show an arterial depression if injected upon the following day with tuberculin.
We have tested several “crude” tuberculins which have been heated in the autoclave at from 7 to 10 lbs. pressure for 30 minutes. The temperatures at these pressures varied approximately from 110° C. to 115° C. We have also tested the unconcentrated filitrate from he tubercular culture when it has reached a condition ready for concentration into “crude” tuberculin. Dogs sensitized with some of the samples of tuberculin which have been heated in the autoclave do not produce the reaction if injected upon the following day with the same tuberculin. Serum of dogs sensitized with the unconcentrated filtrate from the culture of B. tuberculosis of six weeks growth did produce a well marked reaction when injected into the dog on the following day.
Get full access to this article
View all access options for this article.