Abstract
Summary
1. A technic is described for rendering surface and subsurface tissues of amphibia wholly sterile for use in tissue culture. Advantage is taken of the nontoxic selective antibiotic action of small concentrations of sodium sulfadiazine and streptomycin.
2. A medium which supports organogenesis, differentiation and growth of isolated organ and organ system primordia of amphibian embryos has been reported. The principal components of this medium are citrated plasma, salts, agar, glucose and sodium nucleate.
3. Morphogenetic activity in an explanted piece of tissue occurs only when the epidermis heals so as completely to cover the explant.
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