Abstract
Summary
1. Infective Ascaris eggs treated in “Clorox”-sodium hydroxide solution, hatched and produced viable larvae when they were subsequently:
(a) incubated in physiological saline at 37.5° and 40°C. Hatching occurred up to 100% in 4 weeks.
(b) dried, rewet and incubated in saline at 37.5°C. Hatching occurred up to 100% in 2 days.
(c) centrifuged at speeds from 2000 to 3000 R.P.M. Hatching occurred up to 99.1% during 10 minutes of centrifugation at 3000 R.P.M. with a starting temperature of 40°C.
2. Batches of eggs from different worms showed different hatching characteristics depending on the treatment strength and time, and influenced by subsequent procedure.
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