Abstract
Summary
A procedure for the identification and determination of creatine, creatinine, glycocyamidine and glycocyamine is presented. Following resolution by paper chromatography, the developed chromatograms are heated to 100°C for one hour to dehydrate creatine and glycocyamine in situ to creatinine and glycocyamidine respectively. The latter compounds and others of similar structure give an orange color on treatment with the Jaffe reagent (alkaline picrate).
The Voges-Proskauer reaction (alkaline diacetyl) for creatine was also investigated and found suitable but less precise.
RF values for creatine, creatinine, glycocyamine, and glycocyamidine are given for water-saturated solutions of butanol, phenol, and lutidine-collidine (50-50).
The chromatographic method possesses the advantage that creatine and creatinine can be determined independently and directly rather than by a differential procedure as in the conventional methods.
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