Abstract
Summary
Experiments are reported on the effect of methanol and ethanol on the viability and purification of psittacosis virus. A comparison of this technic with differential centrifugation is presented.
The results suggest that the separation of virus from crude allantoic fluid was dependent on a non-specific adsorption of the virus on particulate matter either present originally in the allantoic fluid or brought out of solution by the alcohol. With virus suspensions first purified by centrifugation, the speed of centrifugation and not the precipitation by alcohol was the determining factor in the recovery of virus.
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