Abstract
Summary
Female rabbits were inseminated with semen diluted with Krebs solution at rates of 1/1000 up to 1/5000 and 0.2 cc of suspension were used. The number of spermatozoa effective for partial fertility was 16,000, and for maximum fertility was around 90,000. The group percentage of fertility resulting from vaginal insemination was slightly higher than from uterine insemination (50% vs 40%); this difference was not of statistical significance.
When semen samples were diluted from 1:100 up to 1:12800, the motility of the spermatozoa decreased significantly as the rates of dilution increased and the average motility shown in Krebs solution and the maximal dilution that would support motility was significantly higher than in 0.9% sodium chloride solution.
When females were inseminated with a small number of spermatozoa (20,000) suspended in different volumes of Krebs solution, the average percentages of fertility were higher in the smaller volumes. The motility of the spermatozoa, however, appeared to have a greater effect on the fertility than the dilution.
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