Abstract
Summary
1. A study of the reactions and reaction products of hemin and coordinating substances and certain compounds and conjugates related to them has been made in liquid ammonia.
2. The nomenclature used to describe new compounds and conjugates that are formed in liquid ammonia has been adopted primarily from the nomenclature as used by Keilin for derivatives of hemin and from the nomenclature as used by Franklin in his studies of the nitrogen system of compounds.
3. A new derivative of hemin and liquid ammonia has been described, which exists only in liquid ammonia and which absorbs strongly at 555 mμ.
4. A compound has been prepared by dispersing liquid ammonia treated hemin in water. This compound absorbs at 620 m/x, which is the same spectral region as that found for some hemin compounds occurring in the blood of patients suffering from black water fever. The ammano-hemin is soluble in water at pH 5.5 and it is also soluble in ethylene glycol.
5. A new preparation has been made by combining egg albumin with hemin in liquid ammonia. This conjugate absorbs in two bands at 532 mμ and 565 mμ. These bands lie midway between those obtained by other workers for haem-albumin and serum hemochromogen. Fairley and Keilin were able to prepare haem-albumin only when serum albumin was used.
6. Caffeine hemin can be prepared in liquid ammonia. It absorbs at 577 mμ and 548 mμ, when it is dispersed in liquid ammonia.
7. Insulin reacts with choline chloride and hemin in liquid ammonia to form a conjugate in which the strong absorption band of insulin at 275 mμ is missing, yet this conjugate maintains a prolongation of lowered blood glucose of 5 times the duration obtained by insulin controls.
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