Abstract
Summary
If separated from the tumor cells by centrifugation, the mouse mammary carcinoma extracts lose their hemolytic potency, when incubated at 37° C, after 5 hours. On the other hand, the hemolytic potency of the tumor extracts is either moderately, or substantially increased following incubation in presence of tumor cells, at 37° C, for either 5 or 24 hours.
Fresh extracts prepared from normal mouse cells have in most instances no hemolytic potency, except for those made from active mouse mammary gland. If the normal mouse cell suspensions (liver, lungs, muscle, or pooled organs), however, are incubated at 37° C for 5, or better for 24 hours, some of them may show a slight hemolytic potency, though to a lesser degree than that observed in the case of pre-incubated tumor cell suspensions.
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