Abstract
Summary
Fixation of molecular nitrogen could not be induced in free-living cultures of Rhizobium by supplying them with extracts of root nodules containing the hemoglobinlike pigment. Kine experiments were made during 2 growing seasons. Variations in technique included: species of bacteria; source of nodular extract; addition of oxalacetic, α-ketoglutaric and citric acid; and method of preparing the extract. Analyses of the results showed that gains of 0.2-0.3 mg N were required lor statiitical significance, but even these slight increases were not consistently obtained.
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