Abstract
Summary
Thirty-three doe rabbits were inseminated with a minimal effective number of spermatozoa suspended in saline containing purified testis hyaluronidase, or saline and supernatant fluid of heated normal semen containing hyaluronidase, or in saline and semen of vasectomized buck containing no hyaluronidase. Thirty-two does were inseminated at the same time with the same number of sperms collected from the same rabbit but suspended in saline, serving as parallel controls. It was found that it was the seminal fluid, not hyaluronidase, which really increased the fertilizing capacity of spermatozoa.
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