Abstract
Summary
A strain of Trypanosoma cruzi cultured from experimentally-infected guinea pig on N.N. medium was subcultured at various intervals on N.N. medium for 13 years without animal passage. At the end of this period the strain was found to be still capable of producing infection in experimental animals.
The presence of Tr. cruzi was microscopically demonstrated in the circulating blood of 50% of the inoculated animals. However, cultures made from the heart blood of all the animals on N.N. medium were positive for Tr. cruzi.
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