Abstract
Summary
1. A method for estimation of estrone, estradiol, and estriol in pure solution is based on the observation that these substances afford products with a green fluorescence when heated in phosphoric acid.
2. The fluorescent response of the urinary estrogens is over a wide range a linear function of their concentration. The error of the fluorimetric assay in suitable ranges of estrogen concentrations (estradiol, 0.5-2.5 γ; estrone, 1.0-5.0 γ; and estriol, 1.0-10.0 γ) does not exceed ± 3%.
3. Sterols which do not contain a conjugated double bond, some naturally occurring nonsteroid aromatic substances, and many common constituents of biological material give no fluorescent response under the conditions of the test.
4. Estrogen fluorimetry appears to compare favorably as to sensitivity, specificity and simplicity with previously described methods of estrogen assay.
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