Abstract
Summary
Neither fractional ultrafiltration nor fractional centrifugation have revealed any heterogeneity of size among the particles of the bacteriophage T2K (formerly called PC). The methods used for the measurement of size, namely, diffusion through agar and sedimentation in an angle centrifuge, were essentially qualitative. but the experimental arrangements were such as to yield unequivocal results.
It must be concluded therefore that this bacteriophage has a minimal particle diameter of 60 mμ or more, as has been found previously for unfractionated phages of the same group by a variety of methods.
It is necessary also to withdraw the conclusion previously reached that this bacteriophage has a low infectivity for susceptihle bacteria.
An explanation is suggested for some anomalous results previously obtained in attempts to measure the diffusion of bacteriophage through the porous glass boundary. The use of bacteriophage to measure the rate of flow of fluid as a means of control in the application of this method to other materials is described.
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