Effect of Mustard Gas on Mitosis and P 32 Uptake in Regenerating Liver. ∗

Abstract

Summary

1. Within 3 hours after intravenous injection, 0.1-0.4 mg of βis (β-chloroethyl) sulfide per 50 g rat produces a marked reduction in the number of cells in metaphase and anaphase in regenerating rat liver. A dose of 0.2 mg inhibits mitosis for at least 24 hours. By 48 hours, this inhibition is released. If the mustard reaches the liver before mitosis is initiated the cells are prevented from entering into mitosis.

2. There is no significant change in P³² uptake by liver cells during the interval 3-23 hours after the mustard is injected.

3. Comparison is made with the effects of X-rays on mitosis and P³² uptake. No chromosome abnormalities were observed at the dosage levels used.